Cell Line Lysates
American Research Products Inc PrEST Antigen SRD5A1
PrEST Antigen SRD5A1 antigen APrEST85563 for steroid-5-alpha-reductase alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) Recombinant protein fragment of Human SRD5A1
Cayman Chemical Humn GlucocortIcoId RECPTR
Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. IB00201-32, IB00201, IB00202]
Sino Biological Recombinant Human CNR1 Protein (VLP, Flag-Strep & His tag)
A DNA sequence encoding the Human CNR1 (P21554-1) (Met1-Ser414) was expressed, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. This protein was prepared using our VLP platform.As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Sino Biological Recombinant Human CD9 Protein (Nanodisc, Full-length, His-GFP Tag), Fluorescent
A DNA sequence encoding the Human CD9 (P21926) (Met1-Val228) was expressed with a N-terminal polyhistidine tag followed by a GFP Tag. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Sino Biological Recombinant Human LGR5 Protein (VLP, Flag & His-Strep Tag)
A DNA sequence encoding the Human LGR5 -VLP (O75473) (Gly22-Ser844) was expressed, with a Flag tag at the N-terminus, a polyhistidine tag and a strep tag at the C-terminus.
Sino Biological Mouse Cd1d1 & B2M Heterodimer Protein (His Tag)
A DNA sequence encoding Mouse Cd1d1 (NP_031665.2) (Met1-Gly305) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Mouse B2M (P01887) (Met1-Met119, A105D) constructed the plasmid 2. The two plasmids were co-expressed and the Mouse Cd1d1/B2M heterodimer was purified.
ATCC HeLa S3; Cervical Adenocarcinoma; Human (Homo sapiens)
HeLa S3 cells are a clonal derivative of the parent HeLa cell line (ATCC CCL-2). The HeLa S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability. This line can be adapted to grow in suspension and is a suitable transfection host. The cells are positive for keratin by immunoperoxidase staining. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.